DOUBLE-STRANDED DNA WITH COHESIVE END(S), AND METHOD OF SHUFFLING DNA USING THE SAME

摘要

To provide a method of mutation of DNAs, which is substantially different from the conventional methods applicable to naturally-existing DNAs, and also to provide useful genetic products to be produced by the use of thus-mutated DNAs. A DNA with a cohesive end comprising (a) a double-stranded DNA having the same sequence as that of a part of a gene, and (b) a single-stranded DNA having a base sequence that exists on said gene at the site not adjoining the part corresponding to said double-stranded DNA or a base sequence which said gene does not have, wherein the single-stranded DNA is linked to either one end of the double-stranded DNA to form a cohesive end; a method for producing it; a method of shuffling a DNA using it; a DNA and a DNA pool to be obtained by the shuffling method; a method for producing the DNA pool; and a genetic product to be obtained by expressing the genetic information existing in the DNA pool.