SERUM-FREE MEDIUM FOR GROWTH OF ANCHORAGE-DEPENDANT MAMMALIAN CELLS

摘要

A serum-free medium for growing anchorage-dependent mammalian cells in tissue culture including a basal medium supplemented with components for replacing serum at the concentration range as set forth in Table 1 is disclosed. The tissue culture is stationary or agitated using microcarriers. In an embodiment, the fibronectin is added to the medium immediately prior to use. Alternatively, the fibronectin is used to precoat tissue culture flasks or the microcarriers. In an embodiment, the present invention provides a serum-free medium for growing anchorage-dependent Vero (African green monkey) cells in tissue culture including a basal medium supplemented with the components at the concentration range as set forth in Table 2. The present invention also provides a method of culturing anchorage-dependent mammalian cells, such as Vero cells, in tissue culture including the step of culturing in a basal medium supplemented with the components at the concentration range as set forth in Table 1.