| 摘要 |
<p>The present invention discloses a novel method for treating nucleic acid solutions with alkaline protease substantially to inactivate proteins in the solution, particularly proteins capable of degrading nucleic acid material of interest in the solution. The present invention also discloses a novel method for isolating a nucleic acid material, such as RNA or DNA, from a biological sample by incubating an alkaline lysate of the sample with alkaline protease. In a preferred aspect of the method of isolation of this invention, a resin matrix of silica particle is used to isolate a nucleic acid material from a nucleic acid solution treated with alkaline protease. Application of the disclosed methods to a biological sample or to a nucleic acid solution results in nucleic acid material which is sufficiently free of contamination with active proteins to be useful for a variety of different practical applications.</p> |
