| 摘要 |
The present invention relates to artificial blood and blood substitutes and supplements made by a process for cross-linking a proteinaceous material including an oxygen transport/binding protein such as hemoglobin, methhemoglobin, myoglobin and hemocyanin. The process comprises: i) soaking the proteinaceous material including the oxygen transport/binding protein to be cross-linked in an aqueous solution of high osmolality; ii) incubating the material in an aqueous buffer including an amount of a photooxidative catalyst sufficient to catalyze photooxidation of the material; and iii) irradiating the material and the catalyst of step (i) with light that includes a range of wavelengths selectively absorbed by the catalyst. Irradiation is effected under conditions such that cross-linking of the material occurs.
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