发明名称 |
Synthetic gene coding for human parathyroid hormone |
摘要 |
Disclosed are (1) a DNA containing a synthetic gene for expression of human parathyroid hormone represented by the following DNA sequence: TCTGTG TCCGAGATTC AGTTAATGCA TAACCTTGGC AAACATTTGA ACTCCATGGA GCGTGTAGAA TGGCTGCGTA AGAAGTTGCA GGATGTGCAC AATTTTGTTG CCTTAGGTGC CCCATTGGCT CCTCGTGATG CTGGTTCCCA AAGACCACGT AAAAAGGAAG ACAATGTCTT AGTTGAGAGC CATGAAAAAT CCCTAGGCGA GGCAGACAAG GCCGATGTGA ATGTATTAAC TAAAGCTAAA TCCCAG (2) a method for producing the DNA described in (1), which comprises enzymatically ligating a plurality of oligodeoxynucleotides to one another to form the DNA and inserting the DNA into a vector if necessary, (3) a transformant transformed by the DNA described in (1), and (4) a method for producing human parathyroid hormone which comprises cultivating the transformant described in (3), accumulating human parathyroid hormone in a culture medium, and collecting the same, whereby hPTH can be allowed to express in large amounts in a system using E. coli as a host.
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申请公布号 |
US5714349(A) |
申请公布日期 |
1998.02.03 |
申请号 |
US19960689190 |
申请日期 |
1996.08.05 |
申请人 |
TAKEDA CHEMICAL INDUSTRIES, LTD. |
发明人 |
FUKUDA, TSUNEHIKO;OSHIKA, YURI;YAMADA, TAKAO |
分类号 |
C07K14/635;C12N1/21;C12N15/16;(IPC1-7):C12P21/06;C07H17/00 |
主分类号 |
C07K14/635 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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