Compositions for expression of proteins in host cells using a preprocollagen signal sequence

摘要

Hybrid genes comprising a nucleotide sequence encoding a preprocollagen signal sequence operatively linked to a nucleotide sequence encoding a heterologous protein are disclosed. The hybrid genes are useful for secretion of heterologous proteins from host cells. In a preferred embodiment, the signal sequence of the human preprocollagen alpha 1(I) protein is linked to a heterologous protein of interest to allow for secretion of the protein from Hansenula polymorpha cells.