Method of producing a cell carrying an excess of mammalian centromeres

摘要

DNA fragments and methods for obtaining them are disclosed which when put into mammalian cells together with a dominant marker gene are able to generate functional centromeres. The sequences can be used to form probes for these centromeres. Cell lines containing the functional centromeres are also provided. Methods are taught for isolating mammalian centromeric DNA as well as for producing cell lines carrying an excess of mammalian centromeres linked to a dominant selectable marker gene.