MICROBIAL ISOPRENE GENERATION

摘要

A micro-organism is disclosed, as well as the use of this micro-organism to generate isoprene and a process for producing the same. The isoprene synthase gene of a plant, for example the English oak, is identified and cloned in a micro-organism by means of a gene fragment amplified by appropriate oligonucleotides. The micro-organism that contains an isoprene synthase gene is cultivated and the isoprene released thereby is extracted from the gaseous space above the micro-organism culture by cold fractionation.