The invention concerns a composition containing at least one nucleic acid a part of which at least is enclosed in multilamellar vesicles, characterised in that said vesicles consist of two layers comprising at least one surfactant, the said two layers being concentric and giving said vesicles an onion-like structure. The surfactants used are non-cationic surfactants. The invention concerns the use of such compositions in pharmaceutics, particularly in gene therapy and in in vitro and in vivo transfection.
