SITE-SPECIFIC MUTAGENESIS AND MUTANT SELECTION UTILIZING ANTIBIOTIC-RESISTANT MARKERS ENCODING GENE PRODUCTS HAVING ALTERED SUBSTRATE SPECIFICITY

摘要

Methods, kits, and reagents for conducting site-specific mutagenesis of single or double-stranded nucleic acids which utilizes novel antibiotic resistance conferred by a mutated antibiotic resistance gene for efficient mutant selection are described.