USE OF O-BETA-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS INTENDED TO THE EVALUATION OF INTESTINAL LACTASE, AND PRODUCTION PROCESS

摘要

There is disclosed the use of O- beta -D-galactopyranosyl-D-xyloses, particularly 2-O- beta -D-galactopyranosyl-D-xylose, and 3-O- beta -D-galactopyranosyl-D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. There is also disclosed a process for producing O- beta -D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of beta -D-galactopyranoside in the presence of an enzyme beta -galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37 DEG C; deactivating the beta -galactosidase by heating at 100 DEG C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/ alcohol.