| 摘要 |
A reflecting fluorescence microscope includes an excitation filter for converting a beam of light projected from a reflecting illumination light source into light having a plurality of wavelength regions of narrow-band excitation light, a dichroic mirror for irradiating a specimen with the light having a plurality of wavelength regions of narrow-band excitation light converted by the excitation filter to transmit various kinds of fluorescent light emitted from the specimen, and an absorption filter for absorbing an unwanted wavelength region from the light transmitted through the dichroic mirror to transmit the wavelength of a fluorescent image. In this reflecting fluorescence microscope, at least one band-stop filter having at least one absorption band is disposed in a reflecting illumination optical path before the dichroic mirror so as to absorb the wavelengths of a transmission band and change the amount of the light having a plurality of wavelength regions of narrow-band excitation light. Thus, the fluorescent image which is easy to observe can be obtained.
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