发明名称 |
VERFAHREN ZUR EXTRAKTION, AMPLIFIKATION UND ZUM NACHWEIS VON NUKLEINSÄUREN AUS VOLLBLUT |
摘要 |
A nucleic acid is rapidly extracted from whole blood or a peripheral blood mononuclear cell (PBMC) fraction thereof. Extraction from the PBMC fraction is accomplished by heating the fraction at or near the boiling point of water for a few minutes and recovering the extracted nucleic acid. This rapid method is particularly useful for extracting DNA for the detection of genetic diseases or infectious agents, such as HIV-I. Whole blood can likewise be heated after it is mixed with a salt solution containing a polysaccharide, such as dextran. The extracted nucleic acid is then recovered from the heated mixture. Nucleic acids extracted in this way are available for amplification using a polymerase chain reaction. Where the presence of a specific gene is to be determined for diagnostic purposes, it can be extracted as described above and subjected to suitable amplification and detection steps. |
申请公布号 |
AT162222(T) |
申请公布日期 |
1998.01.15 |
申请号 |
AT19940200344T |
申请日期 |
1990.04.09 |
申请人 |
JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC. |
发明人 |
BURDICK, BRENT ARTHUR;EKEZE, TOBIAS DAVIS |
分类号 |
C07H1/06;C07H21/00;C07K14/16;C07K14/74;C07K14/805;C12N15/10;C12Q1/68;G01N33/50;G01N33/58;(IPC1-7):C12P19/34 |
主分类号 |
C07H1/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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