发明名称 |
Primers labeled with energy transfer coupled dyes for DNA sequencing |
摘要 |
Compositions are provided for making oligonucleotides carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donor at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair, and to have enhanced emission intensities from the acceptor. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.
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申请公布号 |
US5707804(A) |
申请公布日期 |
1998.01.13 |
申请号 |
US19950410808 |
申请日期 |
1995.03.27 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
MATHIES, RICHARD;GLAZER, ALEXANDER;JU, JINGYUE |
分类号 |
G01N33/53;C12Q1/68;G01N33/533;G01N33/542;G01N33/566;G01N33/58;(IPC1-7):C12Q1/68;C07H21/04 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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