发明名称 |
Process for the production and refolding of unfused IGF-II protein in E. coli |
摘要 |
A process for the preparation of a recombinant IGF-II (rIGF-II) without a covalently attached foreign protein moiety and without N-terminal attached methionine or a derivative of methionine or of a salt of said IGF-II, rIGF-II produced by said method, hybrid vectors comprising DNA encoding said rIGF-II, hosts transformed with said vectors, and a process for the isolation of said rIGF-II from the host cell and refolding it into a biologically active form.
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申请公布号 |
US5708148(A) |
申请公布日期 |
1998.01.13 |
申请号 |
US19940341275 |
申请日期 |
1994.11.16 |
申请人 |
CIBA GEIGY CORPORATION |
发明人 |
SCHMITZ, ALBERT;MAERKI, WALTER |
分类号 |
C12N15/02;A61K38/00;A61K38/27;C07K1/113;C07K1/14;C07K1/20;C07K14/575;C07K14/62;C07K14/65;C12N1/21;C12N15/09;C12N15/16;C12N15/17;C12N15/73;C12P21/02;C12R1/19;(IPC1-7):C07K1/14;C12N15/18 |
主分类号 |
C12N15/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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