SOLID PHASE SYNTHESIS OF OLIGONUCLEOTIDES

摘要

Solid phase synthesis of oligeonucleotides The invention therefore relates to a process for preparing oligonucleotides by means of solid phase synthesis by a) sequentially synthesizing the nucleotides on a solid support in accordance with known methods, with exocyclic amino groups which are present on the nucleobases being protected by a cyclic diacyl group and with it being possible to eliminate any phosphate protecting groups which are present with strong nonnucleophilic bases, b) deprotecting the oligonucleotides which are bound to the solid support, and c) cleaving the deprotected oligonucleotides from the solid support in accordance with known methods, which comprises deprotecting the oligonucleotides, which are bound to the solid support, in the presence of a strong, nonnucleophilic base in a suitable organicsolvent.