PROCESS FOR SEPARATING ALPHA-1-PROTEINASE INHIBITOR FROM COHN FRACTION IV1 + IV4 PASTE

摘要

The present invention is directed to a process for purifying alpha-i.PI. The comprises providing an impure protein fraction which comprises alpha-I-PL. The impure protein fraction is suspended in an aqueous solution at pH 6. Insoluble proteins are recovered and resuspended in aqueous solution at pH 8,5. PEG is added to precipitate .alpha.-2 proteins. To the PEG supernatant precipitation, which comprises alpha-1-PI, is added ZnCl2 to precipitate crude alpha-1-PI. The crude alpha-1-PI is resolubilized and applied to an anion-exchange medium. A fraction comprising alpha-1-PI is recovered from the anion-exchang e medium. Alpha-1-PI purified by the process has a specific activity about 1.0 units/OD200. w