OLIGONUCLEOTIDE LINKER AND TECHNIQUES INVOLVING IMMOBILIZED AND LINKED OLIGONUCLEOTIDES

摘要

A technique is provided for linking an oligonucleotide, such as a single strand of DNA, to another moiety or solid phase. An oligonucleotide is provided with a linking functionality that can be defined by six neighboring hystaminyal purines, which can be coordinated to a metal ion that also is coordinated by a chelating linked to a solid phase or other moiety. The invention finds particular use in resolution of one strand of DNA from its complement by providing the first strand with the linking functionality and passing the DNA across a metal affinity chromatography solid phase.