| 摘要 |
A method for synthesis of random biopolymers, particularly oligonucleotides. The method which can randomly incorporate different residues at defined positions with a controllable rate while preserving certain positions as single type residues. The oligonucleotides can be manually or automatically synthesized. For synthesis of oligonucleotides, "site doping synthesis" (SDS) utilizes reservoirs of conventional phosphoramidites and other reservoirs with the same phosphoramidites which have been mixed ("doped") with a mixture of the other phosphoramidites or other nucleotide analogues. The method can be used for mutagenesis with mutation at specific positions. Synthesized oligonucleotides may be used for isolating agonists and antagonists of a protein, such as a protein inhibitor or other therapeutic drug, for producing specified random peptides or proteins, and for developing antisense, antigene and ribozyme therapeutic drugs. |
