| 摘要 |
The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal beta ->4 to GlcNAc or Glc; adding GalNAc or GlcNAc beta 1->3 to Gal; and adding Gal alpha 1->4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal beta 1->4GlcNAc beta 1->3Gal beta 1->4Glc (a mimic of lacto-N-neotetraose), GalNAc beta 1->3Gal beta 1->4GlcNAc beta 1->3Gal beta 1->4Glc beta 1->4 (a mimic ganglioside), and Gal alpha 1->4Gal beta 1->4Glc beta 1->4Hep->R (a mimic of the saccharide portion of globo-glycolipids).
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