发明名称 |
Method to detect hcv specific nucleic acids |
摘要 |
A process to detect hepatitis C virus (HCV) specific nucleic acids comprising the steps of: (a) reverse transcribing the viral RNA by means of a primer having specified sequences; (b) amplifying by a single polymerase chain reaction (PCR) with a specific primer; wherein the Mg<++>/Taq polymerase ratio in the reaction mix is of approx. 100 nmoles/enzyme unit; (c) revealing the amplified product by means of the DEIA method using a specific probe. |
申请公布号 |
AU3047797(A) |
申请公布日期 |
1998.01.05 |
申请号 |
AU19970030477 |
申请日期 |
1997.06.03 |
申请人 |
SORIN BIOMEDICA DIAGNOSTICS S.P.A. |
发明人 |
PAOLO BOSIO;CLAUDIA STRUMIA;FILIPPO CLEMENZA |
分类号 |
C12Q1/70 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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