| 摘要 |
An endonuclease isolated from celery, CEL I, is disclosed as well as methods for use in detection of mutations in targeted polynucleotides. The methods facilitate localization and identification of mutations, mismatches and polymorphisms. The enzyme recognizes every type of mismatch regardless of the sequence context in which the mismatch resides and the enzyme is active in pH ranges from acidic to basic. |
