发明名称 |
METHOD OF IDENTIFYING CYTOCHROME P450 |
摘要 |
A method for identifying a nucleotide sequence encoding a cytochrome P450, the cytochrome being inducible or suppressible in cells of a given type by an agent. The method includes: exposing a first group of the cells to the agent so as to induce or suppress expression of the cytochrome P450; isolating first mRNA from the cells; isolating second mRNA from a second group of the cells which have not been exposed to the agent so as to induce or suppress expression of the cytochrome P450; amplifying the first and second mRNA, respectively, in the presence of an oligo(dT) based first nucleic acid primer sufficient to prime synthesis from a poly(A) tail and a second nucleic acid primer substantially complementary to a nucleic acid sequence encoding a conserved region of a known cytochrome P450; displaying amplified products of the first mRNA and amplified products of the second mRNA to detect differences therebetween; and identifying said nucleotide sequence encoding said inducible or suppressible cytochrome P450. |
申请公布号 |
WO9749832(A2) |
申请公布日期 |
1997.12.31 |
申请号 |
WO1997CA00488 |
申请日期 |
1997.06.23 |
申请人 |
QUEEN'S UNIVERSITY AT KINGSTON;PETKOVICH, P., MARTIN |
发明人 |
PETKOVICH, P., MARTIN |
分类号 |
G01N33/50;A61K38/00;A61K38/44;A61K48/00;C07K16/40;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/02;C12N15/09;C12N15/53;C12P21/02;C12P21/08;C12Q1/68;G01N33/15;G01N33/574;G01N33/577 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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