发明名称 |
Procedure for normalization of cDNA libraries |
摘要 |
This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.
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申请公布号 |
US5702898(A) |
申请公布日期 |
1997.12.30 |
申请号 |
US19950465857 |
申请日期 |
1995.06.06 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
BONALDO, MARIA DEFATIMA;SOARES, MARCELO BENTO |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C12N15/00;C07H21/00 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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