发明名称 |
Probe composition containing a binding domain and polymer chain and methods of use |
摘要 |
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
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申请公布号 |
US5703222(A) |
申请公布日期 |
1997.12.30 |
申请号 |
US19950561478 |
申请日期 |
1995.11.21 |
申请人 |
THE PERKIN-ELMER CORPORATION |
发明人 |
GROSSMAN, PAUL DAVID;FUNG, STEVEN;MENCHEN, STEVEN MICHAEL;WOO, SAM LEE;WINN-DEEN, EMILY SUSAN |
分类号 |
C12Q1/68;(IPC1-7):C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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