| 摘要 |
<p>Disclosed are methods of detecting protein-protein interactions among two populations of proteins, wherein each protein population has a complexity of at least 1,000. Fusion proteins of each population are expressed in yeast cells of opposite mating types. The fusion protein populations are made by fusing to one population a DNA-binding domain of a transcriptional activator and fusing to the other population an activation domain of a transcriptional activator. When the yeast cells of opposite mating types are mated, productive interactions between members of each protein population functionally reconstitute the two domains of the transcriptional activator and result in reporter gene expression. The disclosed methods allow identification and characterization of new protein-protein interactions that may be relevant to a particular tissue or disease stage. Inhibitors of the identified protein-protein interactions can also be identified by screening for the ability to inhibit expression of the reporter gene. This inhibitor screening method can be performed in multiplex. Other aspects of the invention include information processing methods and systems. The methods and systems provide for assembling and processing of a unified database of sequences and identifying sequences that may be involved in protein-protein interactions.</p> |
