| 摘要 |
The present invention is a method of sequencing polynucleotides by sequential step sequencing. Sequential step sequencing begins with a single-stranded polynucleotide template that is annealed with a primer forming a primertemplate complex. In one embodiment of the method, one labeled nucleotide at a time is added to the primer-template complex. Alternatively, this method can be used with a nucleotide or nucleotide analog that does not stop addition after a first nucleotide or nucleotide analog has been added to the primer. Other embodiments of the invention involve identification of polynucleotides having a contiguous non-redundant string or a superimposed non-redundant string pattern. The detection of a non-redundant contiguous string can be used to identify a particular gene. Alternatively, if the non-redundant contiguous string is not unique to a particular gene, the string can be used to form a DNA library that can then be searched with a second string. Similarly, a superimposed non-redundant string pattern can be used to identify a gene or to search a DNA library. Such strings can be used in annealing reactions and in computer searches of a database having a catalog of sequenced polynucleotides. |
