发明名称 |
METHODS AND COMPOSITIONS FOR TRANSFORMING CELLS |
摘要 |
Methods and compositions for transforming cells, resulting in efficient and stable site-specific integration of transgenes, are disclosed. Transformation is achieved by introducing into a cell an acceptor vector, preferably a retroviral vector, which integrates into the genome of the cell. The acceptor vector comprises two incompatible lox sequences, L1 and L2. A donor vector is then introduced into the cell comprising a transgene flanked by the same L1 and L2 sequences. Stable gene transfer is initiated by contacting the lox L1 and L2 sequences with Cre recombinase.
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申请公布号 |
CA2258007(A1) |
申请公布日期 |
1997.12.18 |
申请号 |
CA19972258007 |
申请日期 |
1997.06.06 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY;ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY |
发明人 |
BOUHASSIRA, ERIC;LEBOULCH, PHILIPPE;TAKEKOSHI, KEN JULIAN;WESTERMAN, KAREN |
分类号 |
C12N15/09;C07K14/805;C12N5/10;C12N15/12;C12N15/64;C12N15/867;C12N15/90;(IPC1-7):C12N15/90;A61K48/00;A61K31/70;C12N15/86 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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