摘要 |
<p>PROBLEM TO BE SOLVED: To stably conduct a culture of central neurocytes to enable rapid formation of synapsis excellent in growability of neurite in the case of low- density culture, while to obtain neurocytes high in long-term stability with neuronetwork formed in the case of high-density culture. SOLUTION: This culture liquid is a culture supernatant involved in 1st generation astroglia cells collected after cultured using a nutrient medium incorporated with insulin, transferrin, selenious acid (salt), progesterone, albumin, superoxide dismutase and catalase, or, insulin, transferrin, selenious acid (salt), progesterone, albumin and anα-tocopherol compound.</p> |