发明名称 |
Ribonucleoprotein particles for cleaving double-stranded DNA and inserting an RNA/DNA molecule into the cleavage site |
摘要 |
The present invention provides new methods, employing a nucleotide integrase, for cleaving double-stranded and single stranded DNA substrates at specific sites and for attaching nucleic acid molecules to the cleaved DNA substrates. One method uses a nucleotide integrase to cleave one strand of a double-stranded DNA and to concomitantly attach a nucleic acid molecule to the cleaved strand. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach a nucleic acid molecule to one strand of the DNA substrate. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach an RNA molecule to one strand of the substrate and for attaching a cDNA to the other strand of the substrate. Another method cleaves single stranded DNA with the concomitant insertion of a nucleic acid molecule at the cleavage point. The nucleotide integrase comprises an RNP particle which comprises a group II intron RNA bound to a group II intron encoded protein. The present invention also relates to purified and reconstituted RNP particles and reconstituted RNP that cleave DNA substrates.
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申请公布号 |
US5698421(A) |
申请公布日期 |
1997.12.16 |
申请号 |
US19950526964 |
申请日期 |
1995.09.12 |
申请人 |
THE OHIO STATE RESEARCH FOUNDATION |
发明人 |
LAMBOWITZ, ALAN M.;ZIMMERLY, STEVEN;YANG, JIAN;GUO, HUATAO |
分类号 |
C12N15/09;C12N9/22;C12N15/10;C12Q1/68;(IPC1-7):C12P19/34;C07H21/02;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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