Methods for production of antigens under control of temperature-regulated promotors in enteric bacteria

摘要

Production of proteins in bacteria containing DNA sequences encoding proteins under the control of a temperature-regulated promotor is improved by growing the organisms at temperatures of less than 35 DEG C. until the late logarithmic phase. Thereafter the temperature may be raised to 36 DEG C. to 39 DEG C. Antigens produced by the method of the invention may be used as vaccines, as means for measuring efficacy of vaccines, as probes to detect antigens from clinical samples and for biochemical characterization of antigens.