| 摘要 |
<p>A signal processing method in a processor is provided for performing a multicomponent analysis of a signal resulting from a spectral response of a mixture comprising a plurality of spectrally resolvable molecular species. The method provides both a determination of a concentration estimate and a statistical confidence interval for each species. In the method, a data vector d is received from a multichannel detector, data vector d having a length nc, nc being the number of detector channels being monitored. A calibration matrix K having nc rows and np columns is provided wherein nc is larger than np, np being the number of spectrally resolvable molecular species. Next, a concentration estimate vector c having length np is determined. Finally, a confidence interval CIi for each of the elements of the concentration estimate vector is determined according to the expression CIi = ci ± (varcovar(cii))1/2Q¿(cl, nf)? where Q is the critical value of a statistical distribution for a given level of confidence, cl, and a given number of degrees of freedom nf where nf = nc-np. The invention further includes a program storage device embodying the method; a DNA sequencing process employing the method; and, an apparatus for carrying out the method.</p> |
