METHOD FOR PRODUCING SECRETABLE GLYCOSYLTRANSFERASES
摘要
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferases.
申请公布号
EP0497878(B1)
申请公布日期
1997.12.10
申请号
EP19900916656
申请日期
1990.10.24
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;AMGEN INC.