METHOD OF DETERMINING BILE ACID CONJUGATED WITH SULFURIC ACID AND KIT THEREFOR

摘要

The present invention provides a method of assaying sulfate-conjugated bile acid using bile acid sulfate sulfatase and a reductive system indicator which comprises using an 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt as the reductive system indicator as well as a kit therefor. In accordance with the invention, assaying of sulfate-conjugated bile acid can be performed in a single reaction system using an automated analyzer generally used for clinical examinations, with markedly improved assay operability. The assay is possible using an automated analyzer without requiring much labor. The assay treatment performance of samples can be improved as well.