发明名称 |
PRODUCTION OF GLYCOSIDE BY IMMOBILIZED ENZYME |
摘要 |
PROBLEM TO BE SOLVED: To produce a water-soluble glycoside in high efficiency by reacting a scarcely water-soluble substance having hydroxyl group and exhibiting excellent physiological activity with a sugar in an organic solvent in the presence of an immobilized enzyme produced by immobilizing anα-glucosidase on porous chitosan bead. SOLUTION: The objective water-soluble glycoside having excellent physiological activity such as a chromanol glycoside expressed by formula II [X is glucose residue (H of the hydroxyl group in the glucose residue may be substituted with a 1-8C lower alkyl or a 1-10C lower acyl)] can be produced in high efficiency by reacting a scarcely water-soluble physiologically active substance having hydroxyl group such as a chromanol expressed by formula I (R<1> to R<4> are each H or a lower alkyl; R<5> is H, a lower alkyl or a lower acyl; (n) is an integer of 0-4) with a sugar such as glucose in an organic solvent in the presence of an immobilized enzyme produced by immobilizing 0.01-100mg of anα-glucosidase [based on 1g of porous chitosan bead (wet state)] on a porous chitosan bead having particle diameter of 0.5-3.0mm.
|
申请公布号 |
JPH09313196(A) |
申请公布日期 |
1997.12.09 |
申请号 |
JP19970016756 |
申请日期 |
1997.01.30 |
申请人 |
CCI CORP |
发明人 |
KUNIEDA TSUTOMU;MURASE HIRONORI |
分类号 |
C12N11/02;C07H1/00;C07H15/26;C07H17/065;C12P19/44;(IPC1-7):C12P19/44 |
主分类号 |
C12N11/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|