发明名称 |
Strains of yeast of saccharomyces cerevisiae and a process for the preparation of such strains of yeast |
摘要 |
The present invention relates to a novel strain of the yeast Saccharomyces cerevisiae having accession number MTCC Y0022B211 (=NCYC 2647), which is useful for the preparation of ethanol by the fermentation of sugars. The invention also encompasses a process for the preparation of this strain of yeast, which process comprises: (a) growing a diploid strain of S. cerevisiae designated MTCC Y0001 (=NCYC 2646) in a known medium, sporulating the strain, treating the sporulated cells with a lytic enzyme to liberate the spores, collecting the liberated spores; (b) growing a haploid strain of S. cerevisiae designated MTCC Y0002 (=ATTC 90506); (c) mixing the spores obtained in step (a) with the cells obtained in step (b), incubating the resultant spore-cell mixture at a temperature in the range of 15 DEG C. to 37 DEG C. for a period of 1 to 10 days; (d) spreading the incubated spore-cell mixture on a non-medium, and incubating the mixture at a temperature in the range of 15 DEG to 37 DEG C. for a period of 1 to 10 days; and (e) collecting the cells produced in step (d); and spreading on a selective medium so as to eliminate the spores and cells of steps (a) and (b) and allowing only hybrid cells/cytoductants to grow.
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申请公布号 |
US5693526(A) |
申请公布日期 |
1997.12.02 |
申请号 |
US19960625000 |
申请日期 |
1996.03.29 |
申请人 |
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH |
发明人 |
MONDAL, ALOK KUMAR;PRASAD, GANDHAM SATYANARAYANA;CHAKRABARTI, TAPAN |
分类号 |
C12P7/06;(IPC1-7):C12N1/16 |
主分类号 |
C12P7/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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