Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers

摘要

The present invention relates to an enhanced system for specific, sensitive and rapid detection of the presence and identification of the specific mycoplasma contained in a sample of nucleic acid, wherein the sample nucleic acid is amplified using nested PCR which uses a mixture of first stage and second stage primers, each comprising at least one single sequence and at least one mixed sequence primer.