发明名称 |
METHOD FOR END POINTING ENZYME DETECTION METHODS |
摘要 |
The present invention involves a composition and method for the determination of enzymatic activity or enzyme substrate concentration in a fluid test sample in which the enzyme catalyzes a reaction in a reagent system to provide a detectable response indicative of the concentration of the enzyme or enzyme substrate in the test sample. A measured amount of a protease capable of eliminating the enzymatic activity is included in the reagent composition, so that when a measured amount of test sample is treated, the reaction producing the detectable response will have a definite end point. The invention is particularly useful in conjunction with the determination of leucocytes in urine wherein the leukocytes are lysed to release their human leucocyte elastase which hydrolyzes a chromogenic ester to provide a phenol which then reacts with a diazonium salt to form a colored azo dye.
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申请公布号 |
CA2198951(A1) |
申请公布日期 |
1997.11.28 |
申请号 |
CA19972198951 |
申请日期 |
1997.03.03 |
申请人 |
BAYER CORPORATION |
发明人 |
PUGIA, MICHAEL J.;SCHAEPER, ROBERT J. |
分类号 |
C12Q1/00;C12Q1/26;C12Q1/37;C12Q1/44;C12Q1/54;(IPC1-7):C12Q1/37 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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