发明名称 |
A PROCESS FOR PRODUCING A RECOMBINANT POLYPEPTIDE INVOLVING THE ADDITION OF AN INHIBITOR OF METAL-DEPENDENT PROTEASES OR CHYMOTRYPSINS TO THE CELL CULTURE MEDIUM |
摘要 |
The present invention relates to a process for reducing the detrimental influence of certain proteases on recombinant human protein and polypeptide molecules, by adding an inhibitor of metal-dependent proteases or chymotrypsins to the cell culture medium. The invention also relates to a cell culture medium for cultivating cells expressing and secreting a biologically active recombinant human polypeptide containing an inhibitor of metal-dependent proteases or chymotrypsins, or a combination thereof. The invention further relates to use of recombinant factor VIII which has been produced in a cell culture medium according to the present process for the manufacture of a medicament for administration to a patient having the symptoms of hemophilia A. Also, the invention relates to a method for treatment of hemophilia A by administration of a therapeutically effective amount of recombinant factor VIII which has been produced in a cell culture medium according to the present process.
|
申请公布号 |
WO9743436(A1) |
申请公布日期 |
1997.11.20 |
申请号 |
WO1997SE00783 |
申请日期 |
1997.05.13 |
申请人 |
PHARMACIA & UPJOHN AB;ADAMSON, LARS;WALUM, ERIK;DIXELIUS, JOHAN;LIMA LIE, KRISTINA |
发明人 |
ADAMSON, LARS;WALUM, ERIK;DIXELIUS, JOHAN;LIMA LIE, KRISTINA |
分类号 |
C12N15/09;A61K31/00;A61K38/00;A61K38/43;A61P7/00;A61P7/04;C07K14/755;C12N5/10;C12N9/50;C12N15/67;C12P21/02;(IPC1-7):C12P21/02;C12N5/00;C12N5/16;C12N5/18 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|