| 摘要 |
Disclosed are ribozyme variants and structures making up the catalytic portions of ribozyme variants having enhanced catalytic activity under no turnover conditions in the presence of less than or equal to 10 millimolar concentrations of magnesium. Also disclosed are plasmids encoding such variants, and methods of controlling the expression of a target RNA molecule and of site-specifically cleaving a single-stranded, RNA-containing substrate molecule using such variants. |
