| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new gene, coding for a human RCH1-associated protein having a specific amino acid sequence and useful for the detection of the expression of the gene in various tissues, the diagnosis and treatment of genetic diseases, cancers, etc., through the analyses of the structure and function of the gene, the production of the protein, etc. SOLUTION: A new human RCH1-associated protein gene containing a base sequence coding for an amino acid sequence expressed by the formula. The gene is useful for the detection of the expression of the gene in various tissues, the analyses of its structure and function, the production of a human protein, which the gene codes for, by genetic engineering, and the analysis and diagnosis of a morbid state, the treatment, etc., of related diseases such as genetic diseases and cancers by the analyses of expressed substances, etc. The gene is obtained by preparing a cDNA library using mRNAs extracted from tissues of a human fetus brain, blood vessels of adult, a placenta, etc., according to an ordinary method, selecting positive clones by an immunological cloning method in which proteins, produced by the cDNA when the library is cultured, are made to react with the antibodies specific to the proteins, etc., and recovering the genes by subjecting the positive clones to a restriction enzyme treatment. |
