发明名称 |
Suppression of plant gene expression using processing-defective RNA constructs |
摘要 |
Disclosed is a novel method of suppressing plant gene expression. The suppression is achieved by transforming a plant with a DNA construct encoding a processing-defective RNA (pd-RNA constructs). A pd-RNA construct comprises a plant active promoter operably linked to a pd-RNA encoding segment (pd-RNA segment), wherein the pd-RNA segment comprises a sequence substantially homologous to the target gene and a defective intron and/or a defective 3' termination and processing sequence (hereinafter 3' processing sequence). The pd-RNA constructs of the present invention are designed to express target-gene-homologous RNA transcripts that are defective for messenger RNA processing. Various types of pd-RNA constructs are disclosed, including those defective for endonucleolytic cleavage or polyadenylation at the 3' end of the pd-RNA transcript and/or intron splicing. A pd-RNA construct of the invention may used to suppress a single, specific target gene or multiple target genes. Further, the suppression effect of a pd-RNA construct can be modulated and controlled through the use of an appropriate promoter.
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申请公布号 |
US5686649(A) |
申请公布日期 |
1997.11.11 |
申请号 |
US19950375222 |
申请日期 |
1995.01.19 |
申请人 |
THE ROCKEFELLER UNIVERSITY |
发明人 |
CHUA, NAM-HAI;VAN DER KROL, ALEXANDER |
分类号 |
A01H5/02;C12N15/82;(IPC1-7):A01H4/00;C12N5/14 |
主分类号 |
A01H5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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