| 摘要 |
PROBLEM TO BE SOLVED: To obtain a measuring method in which a plurality of pieces of physicochemical information on a microregion can be made visible simultaneously and easily by a method, wherein molecules are endowed with different functions and the respective molecules are bonded to the surface of a microcarrier. SOLUTION: Polystyrene spheres 20 which contain fluorochrome and whose diameter is at 0.02 to 100μm are dispersed on a substrate 21 such as a glass or the like whose flatness is high, the substrate is installed in a vapor deposition apparatus, gold 22 is vapor-deposited in a vacuum, and a gold thin film in about 10 to 100nm is formed on one face of the spheres. After their vapor deposition, the polystyrene spheres are added to a mixed solution of dithiol which is dissolved in a solvent such as ethanol or the like, and a dithiol molecular layer 24 is formed only on the surface of the gold thin film 23. Then, an antibody 25 which is bonded to a sulfhydryl group modified by colloidal gold or the like is added, the surface of the gold thin film of the spheres is modified, and the spheres in which only one face is modified by an antibody are obtained. When the spheres are used to detect protein on the surface of a cell, fluorescence is observed only from a face opposite to the bonded face of a microcarrier to the surface of a phospholipid double film 31. Thereby, it is possible to obtain not only an antigen-antibody reaction but also orientation information on an antigen- antibody bonded face.
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