Polyclonal and monoclonal antibodies against a 43 KDA dystrophin associated protein

摘要

Disclosed are methods for the preparation of polyclonal and monoclonal antibodies which bind specifically to a 43 kDa dystrophin-associated. The molecular weight of the 43 kDa protein is determined by electrophoretic separation under denaturing conditions, followed by transfer to a solid support and staining with wheat germ agglutinin. The method includes a step in which the peptide PKNMTPYRSPPPYVP (SEQ ID NO: 15) is administered to stimulate an immune response. Also disclosed are polyclonal and monoclonal antibodies which bind specifically to the 43 kDa dystrophin-associated protein.