| 摘要 |
<p>Compounds of general formula (I), wherein each of R?1 and R2¿ independently represents a hydrogen or a halogen atom or another group which does not interfere with subsequent iron chelation; each of R?3 and R4¿ independently represents a hydrogen atom or a (C¿1?-C8)alkyl or (C6 or C10)aryl(C1-C8)alkyl or an optionally modified carboxyl-bearing group of the general formula -CH2(CH2)nCOX, where n is a number from 0 to 3 and X represents a hydroxyl group or another hydrophilic group, and R?3¿ alternatively represents an acyl group of the general formula -COR, in which R represent a (C¿1?-C8)alkyl, (C6 or C10)aryl(C1-C8)alkyl or (C5-C8)cycloalkyl group, provided that R?3 and R4¿ between them contain at least three carbon atoms; or R?3 and R4¿ together with the carbon atoms to which they are attached form a (C¿5?-C8)cycloalkene ring; and one of Y and Z represents an enzymatically cleavable group and the other of Y and Z represents a hydrogen atom; and their salts or hydrates thereof, have been synthesised. The compounds have application in the detection of microorganisms in samples where the compound of general formula (I) is enzymatically cleaved to release a coloured of fluorogenic marker.</p> |
