发明名称 |
METHOD, COMPOSITIONS AND KIT FOR DETECTION AND IDENTIFICATION OF MICROORGANISMS |
摘要 |
<p>Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenaseTM which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide triphosphate feedstocks, and a chain terminating nucleotide triphosphate. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.</p> |
申请公布号 |
CA2252487(A1) |
申请公布日期 |
1997.11.06 |
申请号 |
CA19972252487 |
申请日期 |
1997.04.29 |
申请人 |
VISIBLE GENETICS INC. |
发明人 |
LEUSHNER, JAMES;LARSON, MARINA T.;HUI, MAY;LACROIX, JEAN-MICHEL;DUNN, JAMES M. |
分类号 |
B01L7/00;C12Q1/68;C12Q1/70;G01N35/00;(IPC1-7):C12Q1/68 |
主分类号 |
B01L7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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