| 摘要 |
Modified pyrimidine bases and nucleotides containing a linker arm and a detectable reporter group for nucleic acid labeling. The modified nucleotides have the structure: <IMAGE> wherein: -R1 is -H or -OH; -R2 and -R3 are independently -H, -OH, monophosphate, diphosphate, triphosphate, thio analogs of mono-, di-, or triphosphates, -O- attached to a reactive phosphorous-containing group or -O- protected by a blocking group; -A and -B are independently -H, an electron donating group, an electron withdrawing group, C1-C4 alkyl, halogen, nitro, -COOH, amino, substituted amino, cyano, -CONH, -CSNH, -COOR4 <IMAGE> -CSOR4, -COSR4, -SR4, -COR4, -CH2NHR4, -R4C=CR5H or -C 3BOND C-R4 where R4 and R5 are independently H, an electron donating group, an electron withdrawing group, C1-C4 alkyl, halogen, nitro, COOH, amino, substituted amino, cyano, CONH or CSNH; -X- is -NH-, -NHNH-, -O-, -S-, <IMAGE> or -CH2-; Rep is a detectable reporter group; and -L- is -(CH2)n-(T)q-(CH2)mZ1 or -(CH2)n-(T)q-(CH2)m-Z2-(CH2)pZ1 where n, m and p are each independently an integer from 1 to 20 and q is 1 or 2, -T- is -O-, -S-, <IMAGE> -NH- or a heterocyclic structure containing one or more Group Va or VIa elements as a ring atom, Z1 is a covalent bond, -NH-, -CO-, -O-, -S- or <IMAGE> and -Z2- is -NH-, -NHCO-, -NHCS-, -CONH-, -CSNH-, -O-, -S- or <IMAGE>
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