发明名称 |
Membrane affinity apparatus and purification methods related thereto |
摘要 |
A method and apparatus for carrying out affinity purification of a ligate. The method comprising, (a) providing a ligate containing liquid to a first side of at least one porous hollow fiber membrane with a ligand immobilized thereto that binds and separates the ligate from the liquid, (b) withdrawing a first portion of the liquid from the first side of the porous hollow fiber membrane, (c) recirculating the first portion of liquid to the first side of the porous hollow fiber membrane, (d) repeating steps (a) to (c) until a majority of the liquid has flowed through the porous hollow fiber membrane, and (e) providing an elution solution to one side of the porous hollow fiber membrane under a pressure sufficient to cause the elution solution to flow into and through the membrane to effect disassociation of any ligate-ligand bonds wherein any ligate bound to the ligand is eluted with the elution solution.
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申请公布号 |
US5683916(A) |
申请公布日期 |
1997.11.04 |
申请号 |
US19950465479 |
申请日期 |
1995.06.05 |
申请人 |
HEMASURE INC. |
发明人 |
GOFFE, RANDAL A.;ZALE, STEPHEN E.;O'CONNOR, JAMES L.;KESSLER, STEPHEN B. |
分类号 |
B01D61/00;B01D67/00;B01D69/08;B01D69/14;C07K1/22;C07K16/36;C07K16/40;D01D5/24;G01N30/52;(IPC1-7):G01N33/549;G01N33/543 |
主分类号 |
B01D61/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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