发明名称 |
Mutant AOX2 promoter, vector carrying same, transformant, and production of heterlogous protein |
摘要 |
A mutant AOX2 promoter obtained by mutating a sequence of natural AOX2 promoter in a manner comprising at least one of the three mutation modes of (1) a region extending upstream from nucleotide 1187 inclusive and comprising at least nucleotides 845-960 is deleted, (2) nucleotide(s) is(are) replaced in region(s) in nucleotides 1274-1314, and (3) new oligonucleotide(s) is (are) inserted in region(s) in nucleotides 1274-1314, a vector carrying said mutant AOX2 promoter, a transformant into which said vector has been introduced, and a method for producing a heterologous protein, which comprises cultivating said transformant. The promoter of the present invention has remarkably enhanced activity as compared with natural AOX2 promoter, and is highly useful as a promoter to be carried in an expression vector allowing heterologous protein expression. In addition, the vector and the transformant of the invention can efficiently express and produce various useful heterologous proteins.
|
申请公布号 |
US5683893(A) |
申请公布日期 |
1997.11.04 |
申请号 |
US19950471206 |
申请日期 |
1995.06.06 |
申请人 |
THE GREEN CROSS CORPORATION |
发明人 |
OHI, HIDEYUKI;MIURA, MASAMI;UNO, SHUSEI;CHUGANJI, MASAKO;HIRAMATSU, RYUJI;OHMURA, TAKAO |
分类号 |
C07K14/765;C12N1/19;C12N15/09;C12N15/14;C12N15/81;C12P21/02;C12R1/84;(IPC1-7):C12P21/00;C12N15/64;C07H21/04 |
主分类号 |
C07K14/765 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|