Methods and compositions for the use of HIV env polypeptides and antibodies thereto

摘要

The Human Immunodeficiency Virus envelope protein or its fragments that are capable of binding to the T4 helper lymphocyte receptor are used in therapeutically effective doses for the treatment of immunoinflammatory disorders or diseases. Amino acid residues that constitue an essential portion of the T4 receptor binding domain of HIV env fall within a 64 residue sequence extending about from residues 411 to 454 of the 3B isolate. This domain is useful as a vaccine component, or for cytotoxic T cell targeting when conjugated with a target cell binding substance. HIV env which is devoid of a functional T4 receptor binding domain is useful as a vaccine for immunization against HIV infection. Antibodies capable of binding this domain also are provided for therapeutic and diagnostic use. An immunotoxin comprising a monoclonal antibody to a virally encoded cell surface antigen, linked to a toxin such as ricin A chain, is disclosed. Also, a method of killing virally infected cells such as HIV infected cells, comprising administering to the infected cells a therapeutically effective amount of the immunotoxin is disclosed.